The antibody GF-01 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.

PRODUCT DETAILS

Clone: GF-01

Specificity: GFAP

Reactivity: Human, Feline, Pig

Format: Purified

Isotype: Mouse IgG1

Application: IHC-P, IP, WB, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: human brain (cortex, cerebellum). The antibody GF-01 strongly stains astrocytes in human brain tissue sections but it is essentially negative on mouse and rat tissues.
Immunocytochemistry: Recommended dilution: 5-10 μg/ml.

REFERENCES

1. Brenner M, et al. 2001. Nat Genet 27:117-20.
2. Lukás Z, et al. 1989. Histochem J 21:693-701.

For research use only. Not intended for use in diagnostic procedures.