The mouse monoclonal antibody GA-5 reacts with GFAP, the principal marker of astroglial cells in the central nervous system, which is specifically expressed in satellite cells in peripheral ganglia and in non myelinating Schwann cells in peripheral nerves. The GFAP protein runs on gels at ~55 kDa protein, usually associated with lower Mw bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.

PRODUCT DETAILS

Clone: GA-5

Specificity: GFAP

Reactivity: Human, Pig, Rat

Format: Purified

Isotype: Mouse IgG1

Application: IP, WB, IHC-P, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunocytochemistry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein per 1 ml lysate.

REFERENCES

1. Brenner M, et al. 2001. Nat Genet 27:117-20.
2. Rungger-Brändle E, et al. 2000. Invest Ophthalmol Vis Sci 41:1971-80.
3. Guillemin GJ, et al. 2005. J Neuroinflammation 2:16.
4. Joardar A, et al. 2006. J Lipid Res 47:571-81.
5. Triviño A, et al. 1992. Vision Res 32:1601-7.
6. Perng MD, et al. 1999. J Cell Sci 112 ( Pt 13):2099-112.

For research use only. Not intended for use in diagnostic procedures.