The mouse monoclonal antibody clone GARP5 binds to human GARP, also known as LRRC32 or Garpin, an 80 kDa transmembrane protein that acts as a receptor for the latent form of TGF-beta 1 (pro-TGF-beta), preventing its secretion. Specifically, GARP is reported to associate with the pro-domain of TGF-beta 1, known as latency-associated peptide (LAP), which must be cleaved to release the biologically active cytokine. The putative role of GARP in sequestering the TGF-beta is important for regulating its activity, as TGF-beta signaling is involved in the development and function of Th17, Treg, and many other immune cell types. GARP expression has been shown on platelets and at high levels on Foxp3+ Treg cells, where it is proposed to be a phenotypic identifier for activated human Treg cells.

PRODUCT DETAILS

Clone: GARP5

Specificity: GARP

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: WB, FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: human thrombocytes, reducing conditions

For research use only. Not intended for use in diagnostic procedures.