The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.

PRODUCT DETAILS

Clone: DC-10

Specificity: Cytokeratin 18

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: IP, WB, IHC-P, ICC, ELISA, FC-IC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 1-2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: colon, skin.
Immunocytochemistry: positive control: MCF-7 human breast adenocarcinoma cell line, HeLa human cervix carcinoma cell line.
ELISA: Positive control: MCF-7 human breast adenocarcinoma cell line; the antibody DC-10 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

REFERENCES

1. Cap J, et al. 2003. J Clin Neurosci 10:444-8.
2. Taylor-Papadimitriou J, et al. 1989. J Cell Sci 94 ( Pt 3):403-13.
3. Lauerová L, et al. 1988. Hybridoma 7:495-504.
4. Kovarík J, et al. 1988. Int J Cancer Suppl 3:50-5.
5. Vojtĕsek B, et al. 1989. Folia Biol (Praha) 35:373-82.

For research use only. Not intended for use in diagnostic procedures.