The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

PRODUCT DETAILS

Clone: CRIS1

Specificity: CD5

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: FC, IP, WB, ELISA

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

ELISA: The antibody CRIS1 can be used in the sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32
Flow cytometry: Recommended dilution: 1 μg/ml; positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line.
Western blotting: Laurylmaltoside lysing buffer; non-reducing conditions; recommended dilution: 1-2 μg/ml.

REFERENCES

1. Freedman AS, et al. 1989. Eur J Immunol 19:849-55.
2. Raman C. 2002. Immunol Res 26:255-63.
3. Guarné A, et al. 1996. Protein Sci 5:167-9.
4. Arrizabalaga P, et al. 1989. Nephron 53:41-9.
5. Alberola-Ila J, et al. 1992. J Immunol 148:1287-93.

For research use only. Not intended for use in diagnostic procedures.