The mouse monoclonal antibody clone WM53 binds to human CD33, a 67 kDa type I transmembrane glycoprotein in the immunoglobulin family. CD33, also known as Siglec-3, glycoprotein 67 (gp67), and p67, is expressed on monocytes, activated T cells, myeloid progenitors, granulocytes, dendritic cells, and mast cells. It is absent on erythrocytes, platelets, and lymphoid cells. CD33 binds to sialic acids and acts as a sialic acid-dependent cell adhesion molecule. CD33 also contains intracellular tyrosine-based inhibition motifs (ITIMs), suggesting it functions to inhibit cellular activity.

PRODUCT DETAILS

Clone: WM53

Specificity: CD33

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1, k

Application: FC, IP, WB, ICC, MC-CyTOF

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Immunohistochemistry (frozen sections): Acetone fixation.
Flow cytometry: Recommended dilution: 1-4 µg/ml

For research use only. Not intended for use in diagnostic procedures.