The mouse monoclonal antibody B2M-01 reacts with beta2-microglobulin (beta2M; extracellular antigen), associated with cell-surface MHC Class I molecules and other membrane antigens, as well as it reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.

PRODUCT DETAILS

Clone: B2M-01

Specificity: Beta-2 Microglobulin

Reactivity: Human

Format: Purified

Isotype: Mouse IgG2a

Application: FC, IP, WB, IHC-P, ICC, ELISA, RIA

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 2-4 μg/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: kidney glomeruli.
Flow cytometry: Recommended dilution: 0.5-4 μg/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes.
ELISA: Working dilution should be determinated by investigator.
RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIA.
Immunoprecipitation: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.

REFERENCES

1. Poláková K, et al. 2013. Leuk Res 37:139-45.
2. Khurana M, et al. 2006. Pediatr Nephrol 21:1257-65.
3. Hilgert I, et al. 1984. Folia Biol (Praha) 30:369-76.
4. Polakova K, et al. 1993. Mol Immunol 30:1223-30.

For research use only. Not intended for use in diagnostic procedures.