The mouse monoclonal antibody AFP-11 reacts with human alpha-Fetoprotein (AFP), a 70 kDa oncofetal antigen. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus.

PRODUCT DETAILS

Clone: AFP-11

Specificity: Alpha-Fetoprotein

Reactivity: Human

Format: Purified

Isotype: Mouse IgG1

Application: IHC-P, ELISA, RIA, WB, ICC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100).
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.
Western blotting: Recommended dilution: 2 μg/ml, non-reducing conditions.

REFERENCES

1. Stefanová I, et al. 1988. J Immunol Methods 111:67-73.

For research use only. Not intended for use in diagnostic procedures.