The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.

PRODUCT DETAILS

Clone: IVA285-1

Specificity: Ig Light Chains

Reactivity: Bovine, Ovine

Format: Purified

Isotype: Mouse IgG1

Application: WB, FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Western blotting: Recommended dilution: 0,5-1 μg/ml, overnight at 4°C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12% separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM.
Flow cytometry: Recommended dilution: 1-4 µg/ml

REFERENCES

1. Antalíková J, et al. 2006. Hybridoma (Larchmt) 25:309-12.

For research use only. Not intended for use in diagnostic procedures.