The mouse monoclonal antibody clone W6D3 binds to human CD15, a 220-kDa carbohydrate structure. CD15 is also known as 3-fucosyl-N-acetyllactosamine (3-FAL), Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is highly expressed on granulocytes, including neutrophils, eosinophils, and some on monocytes. It has also been shown to be expressed on Langerhans cells. It is absent from lymphocytes or basophils. CD15, a marker for human myeloid cells, is involved in various cell functions, including phagocytosis, bacterial activity, neutrophil adhesion to dendritic cells, and chemotaxis.

PRODUCT DETAILS

Clone: W6D3

Specificity: CD15

Reactivity: Human

Format: PE-Cyanine7

Isotype: Mouse IgG1, k

Tested Dilution: 5 µL/Test

Application: FC

Storage: 2-8°C protected from light.Do not freeze

Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Flow cytometry: The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.

For research use only. Not intended for use in diagnostic procedures.

PE-Cyanine7 is a tandem dye made with PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.