The mouse monoclonal antibody clone 5C3 binds to human CD40, a 45–50 kDa type I transmembrane glycoprotein and member of the tumor necrosis factor receptor (TNFR) superfamily, also known as TNFRSF5. CD40 is constitutively expressed on B lymphocytes at all stages of development except terminally differentiated plasma cells, and is also present on follicular dendritic cells, monocytes, macrophages, thymic epithelium, a subset of peripheral T cells, and some non-hematopoietic cells including endothelial cells and fibroblasts. CD40 functions as a receptor for CD154 (CD40L, gp39), which is predominantly expressed on activated CD4+ T helper cells. The CD40–CD154 interaction plays an essential role in T cell-dependent B cell activation, driving germinal center formation, immunoglobulin isotype class-switching, memory B cell development, and protection of B cells from apoptosis. Clone 5C3 has been validated for flow cytometric analysis of human peripheral blood mononuclear cells and has been reported for use in immunohistochemical staining of frozen tissue sections and in vitro B cell activation functional assays.

PRODUCT DETAILS

Clone: 5C3

Specificity: CD40

Reactivity: Human, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque

Format: cFluor^® V715

Isotype: Mouse IgG1, k

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Elgueta, R. et al. 2009. Immunol. Rev. 229: 152–172.
2. Karnell, J.L. et al. 2019. Adv. Drug Deliv. Rev. 141: 92–103.
3. Banchereau, J. et al. 1994. Annu. Rev. Immunol. 12: 881–922.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® V715 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Cytek^® cFluor^® V715 is StarBright^™ Violet 710 Dye, manufactured and provided by Bio-Rad, Inc.

StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.