The 2H7 antibody reacts with human CD20 that is expressed by pre-B cells, resting and activated B cells, follicular dendritic cells, and a small subset of T cells, but is absent on plasma cells. CD20 is a 33-37-kDa transmembrane protein which is differentially phosphorylated in resting and activated B cells. The CD20 molecule plays a role in regulating B cell activation. The 2H7 antibody is reported to be cross-reactive with several nonhuman primate species including baboon, chimpanzee, cynomolgus, and rhesus monkeys. Please choose the appropriate format for each application.
The antibody was purified by affinity chromatography and conjugated to the fluorophore.

PRODUCT DETAILS

Clone: 2H7

Reactivity: Human

Format: cFluor^® V515

Isotype: Mouse IgG2b

Tested Dilution: 5 μL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze.

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)

Data Sheets: TDS SDS

EXAMPLE DATA

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For Research Use Only. Not intended for use in diagnostic procedures.

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