cFluor® UV440 anti-Human CD3 (UCHT1)
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説明
The UCHT1 monoclonal antibody binds to the human epsilon chain of the CD3 antigen/T cell antigen receptor, a 20- to 30-kDa protein complex. This complex contains a CD3γ, a CD3δ, a CD3ζ (CD247), two CD3ε, and a T cell receptor (α/β or γ/δ) heterodimer (Dong, D., et al. 1981. Nature. 573, 546–552). CD3 is found on all mature T cells, NKT cells, and thymocytes. It plays a role in recognizing antigen, activating cytotoxic T cells and T helper cells, and signal transduction (Weiss A, et al. 1991. Semin Immunol. (5):313-24).
The antibody was purified by affinity chromatography and conjugated to the fluorophore.
PRODUCT DETAILS
Clone: UCHT1
Reactivity: Human
Format: cFluor® UV440
Isotype: Mouse IgG1
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from light. Do not freeze.
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)
EXAMPLE DATA
cFluor® UV440 anti-Human CD3 (UCHT1), (right) vs. Isotype Control (left). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum.
RECOMMENDED USAGE
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
For Research Use Only. Not intended for use in diagnostic procedures.
*Cytek® cFluor® UV440 is StarBright™ UltraViolet 445 Dye, manufactured and provided by Bio-Rad, Inc.
StarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.