The X54-5/7.1 monoclonal antibody binds to CD64, a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The X54-5/7.1 antibody reacts with mouse strains carrying CD64a and b alleles but not CD64d. X54-5/7.1 recognizes a conformational determinant formed between domains 2 and 3. CD64 binds IgG immune complex, and CD64 expression can be upregulated by IFN-γ stimulation. CD64 plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC). The antibody was conjugated to a fluorophore and purified by chromatography.

PRODUCT DETAILS

Clone:         X54-5/7.1

Reactivity:    Mouse

Format:          cFluor^® BYG575

Isotype:              Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE 

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Benitez B, et al. 2021. Br J Pharmacol 178:4368-4388
2. Xu J, et al. 2020. Immunity 53:371-383
3. Yoshida H, et a. 2019. Cell 176:897-912

For Research Use Only. Not for use in diagnostic or therapeutic procedures.