The L243 monoclonal antibody binds to the human HLA-DR antigen, a 36-kDa alpha heavy chain and a 27- kDa beta light chain heterodimeric cell surface glycoprotein in transmembrane major histocompatibility complex 2 (MHC II) family⁽¹⁾⁽²⁾. HLA-DR, also known as human leukocyte antigen DR isotype, is present on the surface of antigen-presenting cells, including B cells, dendritic cells, macrophages, monocytes and activate T cells. MHC class II regulates the immune system by playing a critical role in binding and presenting antigen-derived peptides to peptide-MHC II-specific CD4 T cells ⁽³⁾. The antibody was conjugated to a fluorophore and purified by chromatography.

 PRODUCT DETAILS

Clone:          L243

Reactivity:     Human, African Green, Baboon, Chimpanzee, Cynomolgus, Dog (Canine), Marmoset, Rhesus, Squirrel Monkey, Tamarin

Format:        cFluor^® R840*⁺

Isotype:            Mouse IgG2a

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

 

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

 

REFERENCES

1. Korman, A J et al. 1982. Proc Natl Acad Sci U S A. 79(19):6013-6017

2. Shackelford DA, et al. 1982. Immunol Rev. 66:133-87

3. Walseng E, et al. 2008. J Biol Chem. 23;283(21):14717-27

*cFluor® R840 contains CF®790 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.

 

⁺cFluor® R840 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.