The Armenian hamster monoclonal antibody clone eBio GL3 binds to mouse T cell receptor (TCR) γδ. The TCR is a heterodimer consisting either of an α and a β chain (TCR α/β) or of a γ and a δ chain (TCR γ/δ). TCR δ belongs to the immunoglobulin superfamily, which recognizes certain bacterial and tumor antigens bound to MHC class I. TCR γδ associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, the peripheral lymphoid tissues, and the peritoneum. Most γδ T cells are CD4- /CD8- although some can be CD8+. T cells expressing the TCR γδ have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that T γδ+ cells also play a principal role in antigen presentation. The clone eBio GL3 reacts with the gamma delta T-cell Receptor complex (TCR) on all gamma delta TCR-bearing T cells, but it does not react with alpha beta TCR. The gamma delta TCR is present on cells in the thymus, epidermis, epithelial lining of the intestine, peritoneal cavity, and lymphoid tissues.

PRODUCT DETAILS

Clone: eBio GL3

Specificity: TCR γ/δ

Reactivity: Mouse

Format: cFluor^® R780

Isotype: Armenian Hamster IgG

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® R780 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.