The mouse monoclonal antibody clone 10.1 binds to human CD64 (FcRI), a 75 kDa type I transmembrane protein. CD64 functions as a high-affinity receptor for IgG, facilitating antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and cytokine production. CD64 is expressed on monocytes, macrophages, dendritic cells, and activated granulocytes, while mature granulocytes and lymphocytes lack expression. Increased expression of CD64 on neutrophils may be indicative of an active immune response to bacterial infection or sepsis in research applications. The 10.1 clone reportedly blocks the binding of immunoglobulin to FcRI.

PRODUCT DETAILS

Clone: 10.1

Specificity: CD64

Reactivity: Human

Format: cFluor^® R780

Isotype: Mouse IgG1

Tested Dilution: 5 µl/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Douglas JL, et al. 2009. J Virol. 83: 7931-7947.
2. Cao W, et al. 2009. J. Exp. Med. 206:1603-1614.
3. Neil SJ, et al. 2008. Nature 451:425-430.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® R780 is a tandem dye made with APC. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.