The dG9 monoclonal antibody binds to human perforin, a 70-kDa pore forming cytolytic protein. Perforin is found in the granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells ⁽¹⁾. Perforin plays a key role in targeted cell lysis by polymerizing to form a channel in target cell membrane, which allows cytotoxic protease like granzyme B to enter the cell ⁽²⁾⁽³⁾. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                dG9

Reactivity:         Human, Cross-Reactivity: Bovine (Cow, Cattle)

Format:             cFluor^® R720*

Isotype:             Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

REFERENCES

1. Lichtenheld MG, et al. 1988. Nature. 29;335(6189):448-51
2. Osińska, Iwona et al. 2014. Central-European journal of immunology vol. 39,1 (2014): 109-15
3. Spicer BA, et al. 2017. Semin Cell Dev Biol. 72:117-123

These products are for research use only. Not intended for use in diagnostic procedures.

*cFluor™ R720 is equivalent to CF®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.