The S-HCL-3 monoclonal antibody binds to human CD11c, a 145-150-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily. CD11c, also known as integrin alpha X, is expressed in high levels on dendritic cells. The molecule is also expressed on monocytes, macrophages, neutrophils, NK cells and subsets of T and B cells. CD11c heterodimerizes with Integrin beta 2 chain to form CD11c/CD18 complex (CR4, complement receptor type 4)¹. This complex binds to fibrinogen, CD54, and iC3b to play a role in leukocyte adhesive interactions².

The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone: S-HCL-3

Specificity: CD11c

Reactivity: Human

Format: cFluor^® R685*

Isotype: Mouse IgG2b, κ

Tested Dilution: 5 µL / test

Application: Flow Cytometry

Storage: 2-8°C and protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES
1. Sándor N, et al. 2016. PloS one vol. 11,9 e0163120
2. Stacker SA and Springer TA. 1991. J Immunol. 146(2):648

For research use only. Not intended for use in diagnostic procedures.

*cFluor^® R685 is equivalent to CF^®660C manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.