The 15C4 monoclonal antibody binds to the human lectin-like oxidized LDL receptor 1 (LOX-1), also known as CLEC8A. LOX-1 is a 31 kD single pass type II transmembrane protein and member of the C-type lectin superfamily, and is a scavenger receptor that is involved in endocytosis, phagocytosis and cytokine production. LOX-1 is expressed by endothelial cells, smooth muscle cells, platelets, fibroblasts, macrophages, and is upregulated by inflammatory and oxidative stimuli. LOX-1 recognizes numerous ligands,  including lipoproteins, apoptotic cells, heat shock proteins, C-reactive protein, and oxidized LDL. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

 PRODUCT DETAILS

Clone:       15C4

Reactivity:     Human

Format:       cFluor^® R659

Isotype:          Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Li D., et al. 2012. J Exp Med. 209:109-121.

2. Yin W., et al. 2016. eBioMedicine 5:46-58.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.