The 6H6 monoclonal antibody binds to human CD123, a 70-kDa type I transmembrane glycoprotein in the immunoglobulin superfamily, also known as the Interleukin-3 receptor alpha chain. CD123 alone has low affinity to IL-3 and develops high affinity when associated with βc (common chain)¹. The CD123 molecule is expressed predominantly on hematopoietic progenitor cells, macrophages, dendritic cells, basophils, eosinophils, monocytes, megakaryocytes and some B cells^2,3. It is also present in myeloid, lymphoid, and vascular endothelial cells⁴. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:            6H6

Reactivity:       Human

Format:          cFluor® BYG610¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Broughton SE, et al. 2014. Cell Rep. 8:410
2. Macardle PJ, et al. 1996. Cell Immunol. 168:59
3. Yamada T, et al. 1998. J Allergy Clin Immunol. 101:677
4. Sun Q, et al.1996. Blood. 87:83

These products are for research use only. Not intended for use in diagnostic procedures.

¹cFluor® BYG610 is a tandem dye made with R-PE. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.