The rat monoclonal antibody clone IM7 binds to human and mouse CD44, a ubiquitously expressed cell surface receptor important for extracellular matrix organization, cell-cell and cell-matrix adhesion, and migration. CD44 may be expressed in several different isoforms (splice variants) from the most typical or “standard” form, CD44s, to variants designated CD44v, e.g. CD44v1 or CD44v6. These receptors interact with several ligands but are most often associated with an extracellular matrix component, hyaluronate, through which it mediates adhesion. The IM7 antibody can be used to identify and quantify all isoforms of mouse CD44, as it recognizes constant epitopes near the extracellular proximal domain.

PRODUCT DETAILS

Clone: IM7

Specificity: CD44

Reactivity: Human, Mouse, Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus Macaque, Equine, Bovine, Pig, Dog, Feline

Format: cFluor^® B690

Isotype: Rat IgG2b, k

Tested Dilution: 5 µL/Test

Application: Flow Cytometry

Storage: 2-8°C protected from light. Do not freeze

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA)

Data Sheets: TDS | SDS

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

REFERENCES

1. Lesley J, Hyman R, et al. Adv Immunol. 1993;54:271-335.

For research use only. Not intended for use in diagnostic procedures.

cFluor^® B690 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.