{"product_id":"tslp-receptor-monoclonal-antibody-ebio1a6-1a6-super-bright-780-ebioscience","title":"TSLP Receptor Monoclonal Antibody (eBio1A6 (1A6)), Super Bright 780, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2a, lambda\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eeBio1A6 (1A6)\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eSuper Bright 780\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e5 µL (0.5 µg)\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e5 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=78-5499-42\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The eBio1A6 monoclonal antibody reacts with human thymic stromal-derived lymphopoietin receptor (TSLPR). TSLPR is an approximately 50 kDa protein with significant similarity to the common gamma-chain. TSLPR complexes with IL-7R alpha (CD127) to form the high affinity receptor that binds thymic stromal-derived lymphopoietin (TSLP). Human TSLPR is expressed by monocytes and CD11c+ dendritic cells, and TSLP binding induces the expression of the Th2 cell-attracting chemokines CCL17 and CCL22. Furthermore, the TSLPR-induced activation of dendritic cells indirectly results in the increased secretion of Th cytokines IL-4, -5 and -13, which may be necessary for the regulation of CD4+ T cell homeostasis. In mice, deficiency of TSLPR has no effect on lymphocyte numbers, whereas double deficiency of TSLPR and common gamma-chain results in fewer lymphocytes than seen in mice deficient in the common gamma-chain alone. The eBio1A6 monoclonal antibody is able to cross-block binding of another anti-human TSLPR monoclonal antibody, 1D8.\u003c\/p\u003e\u003cp\u003eApplications Reported: This eBio1A6 (1A6) antibody has been reported for use in flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This eBio1A6 (1A6) antibody has been pre-titrated and tested by flow cytometric analysis of TSLPR transfected cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003eSuper Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780\/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.\u003c\/p\u003e\u003cp\u003eWhen using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/SB-4401-42\"\u003eProduct # SB-4401\u003c\/a\u003e) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.\u003c\/p\u003e\u003cp\u003eIn some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450\/50 channel when using UltraComp eBeads microspheres (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/01-2222-42\"\u003eProduct # 01-2222-42\u003c\/a\u003e) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A10497\"\u003eProduct # A10497\u003c\/a\u003e).\u003c\/p\u003e\u003cp\u003eLight sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.\u003c\/p\u003e\u003cp\u003eFixation: Samples can be stored in IC Fixation Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-8222-49\"\u003eProduct # 00-8222\u003c\/a\u003e) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix\/Lyse Solution (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5333-54\"\u003eProduct # 00-5333\u003c\/a\u003e) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency\/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.\u003c\/p\u003e\u003cp\u003eExcitation: 405 nm; Emission: 780 nm; Laser: Violet Laser\u003c\/p\u003e\u003cp\u003eSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43191031463972,"sku":"78-5499-42","price":476.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/78-5499-TSLPR-flow-cytometry-30APR2018LSRFKE-eBio1A6-SB780_20180507143340.jpg?v=1769046205","url":"https:\/\/cytekbio.com\/products\/tslp-receptor-monoclonal-antibody-ebio1a6-1a6-super-bright-780-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}