{"product_id":"purified-anti-mammal-vimentin-vi-01","title":"Purified Anti-Mammal Vimentin (VI-01)","description":"\u003cp\u003eThe antibody VI-01 reacts with vimentin, a 57 kDa intermediate filament intracellular protein expressed in variety of mesenchymal and mesodermal cell types. Cross-reactivity was found with smooth muscle desmin.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: VI-01\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Vimentin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Mammal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgM\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: IHC-P, WB, ICC, FC-IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91241+Rev.+A_TDS_Purified+Anti-Mammal+Vimentin+(VI-01).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eImmunocytochemistry: Staining technique: (a) fix cells  for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: 3T3 murine Swiss albino fibroblast cell line, RBL rat basophilic leukemia cell line.\u003cbr\u003eFlow cytometry: Recommended dilution: 1-5 µg\/ml. Intracellular staining.\u003cbr\u003eWestern blotting: Recommended dilution: 1 µg\/ml.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eLukás Z, et al. 1989. Histochem J 21:693-701.\u003c\/li\u003e\n\u003cli\u003eLukás Z, et al. 1993. Histochemistry 100:495-502.\u003c\/li\u003e\n\u003cli\u003eBacáková L, et al. 2000. J Biomed Mater Res 49:369-79.\u003c\/li\u003e\n\u003cli\u003eBacáková L, et al. 2000. Biomaterials 21:1173-9.\u003c\/li\u003e\n\u003cli\u003eDráberová E, et al. 1986. Folia Biol (Praha) 32:295-303.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679927205924,"sku":"R7-91241","price":212.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91241_FC_Histogram.png?v=1792021605","url":"https:\/\/cytekbio.com\/products\/purified-anti-mammal-vimentin-vi-01","provider":"Cytek Biosciences","version":"1.0","type":"link"}