{"product_id":"purified-anti-human-mouse-gamma-tubulin-tu-30","title":"Purified Anti-Human\/Mouse Gamma-Tubulin (TU-30)","description":"\u003cp\u003eThe antibody TU-30 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the amino acid sequence TRPDYI (aa439-444 in human), which is present on human gamma-tubulin 1 but not on human gamma-tubulin 2.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: TU-30\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Gamma-Tubulin\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse, Bovine, Chicken, Pig, Plants, Protozoa, Rat\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: ICC, WB, FC-IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91553+Rev.+A_TDS_Purified+Anti-HumanMouse+Gamma-Tubulin+(TU-30).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eImmunocytochemistry: Recommended dilution: 1-2 μg\/ml. Staining technique: (a) Fix cells  for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.\u003cbr\u003eWestern blotting: Recommended dilution 1-2 μg\/ml, reducing conditions.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eWiese C, et al. 2006. J Cell Sci 119:4143-53.\u003c\/li\u003e\n\u003cli\u003eHaren L, et al. 2006. J Cell Biol 172:505-15.\u003c\/li\u003e\n\u003cli\u003eSulimenko V, et al. 2006. J Immunol 176:7243-53.\u003c\/li\u003e\n\u003cli\u003eWiese C, et al. 2006. J Cell Sci 119:4143-53.\u003c\/li\u003e\n\u003cli\u003eDráberová L, et al. 1999. Int Immunol 11:1829-39.\u003c\/li\u003e\n\u003cli\u003eHaren L, et al. 2006. J Cell Biol 172:505-15.\u003c\/li\u003e\n\u003cli\u003eČernohorská M, et al. 2016. Biochim Biophys Acta 1863:1282-97.\u003c\/li\u003e\n\u003cli\u003eKatsetos CD, et al. 2006. J Neuropathol Exp Neurol 65:465-77.\u003c\/li\u003e\n\u003cli\u003eDráberová E, et al. 2017. FASEB J 31:1828-1846.\u003c\/li\u003e\n\u003cli\u003eBinarová P, et al. 2000. Plant Cell 12:433-42.\u003c\/li\u003e\n\u003cli\u003eLibusová L, et al. 2004. Exp Cell Res 295:375-86.\u003c\/li\u003e\n\u003cli\u003eBinarová P, et al. 2000. Plant Cell 12:433-42.\u003c\/li\u003e\n\u003cli\u003eKlebanovych A, et al. 2019. Cells 8.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679920685092,"sku":"R7-91553","price":226.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91553_FC_Histogram.png?v=1792021500","url":"https:\/\/cytekbio.com\/products\/purified-anti-human-mouse-gamma-tubulin-tu-30","provider":"Cytek Biosciences","version":"1.0","type":"link"}