{"product_id":"purified-anti-human-mouse-cd3-epsilon-activation-epitope-apa1-1","title":"Purified Anti-Human\/Mouse CD3 Epsilon (Activation Epitope) (APA1\/1)","description":"\u003cp\u003eThe mouse monoclonal antibody APA1\/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1\/1 provides the earliest known marker for TCR-mediated T cell activation.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: APA1\/1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD3 Epsilon (Activation Epitope)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: IP, WB, ICC, FC-IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91691+Rev.+A_TDS_Purified+Anti-HumanMouse+CD3+Epsilon+(Activation+Epitope)+(APA11).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry: Intracellular staining; recommended dilution: 1-4 µg\/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg\/ml). Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice.  Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice.  Incubate with fluorescence-labeled APA1\/1 antibody (1-2 μg\/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. \u003cbr\u003eImmunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eRisueño RM, et al. 2006. Proc Natl Acad Sci U S A 103:9625-30.\u003c\/li\u003e\n\u003cli\u003eAlarcón B, et al. 1991. EMBO J 10:903-12.\u003c\/li\u003e\n\u003cli\u003eGil D, et al. 2005. J Exp Med 201:517-22.\u003c\/li\u003e\n\u003cli\u003eRisueño RM, et al. 2005. Blood 106:601-8.\u003c\/li\u003e\n\u003cli\u003eDelgado P, et al. 2000. Nature 406:426-30.\u003c\/li\u003e\n\u003cli\u003eTailor P, et al. 2008. J Immunol 181:243-55.\u003c\/li\u003e\n\u003cli\u003eRieux-Laucat F, et al. 2006. N Engl J Med 354:1913-21.\u003c\/li\u003e\n\u003cli\u003eTorres PS, et al. 2003. J Immunol 170:5947-55.\u003c\/li\u003e\n\u003cli\u003eWong AP, et al. 2007. Am J Physiol Lung Cell Mol Physiol 293:L740-52.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679920029732,"sku":"R7-91691","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91691_FC_Profile.png?v=1792021491","url":"https:\/\/cytekbio.com\/products\/purified-anti-human-mouse-cd3-epsilon-activation-epitope-apa1-1","provider":"Cytek Biosciences","version":"1.0","type":"link"}