{"product_id":"purified-anti-human-cd45rb-mem-143","title":"Purified Anti-Human CD45RB (MEM-143)","description":"\u003cp\u003eThe antibody MEM-143 reacts with an extracellular epitope of CD45RB, a 180-240 kDa single chain type I membrane glycoprotein, variant of CD45 (CD45RB isoform). CD45RB is expressed on a subset of T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells. The MEM-143 is therefore not neuraminidase sensitive like most other anti-CD45RB antibodies (including standard antibody MEM-55). The reactivity of the antibody MEM-143 can be blocked by a peptide including amino acids 79-88.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: MEM-143\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD45RB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC, IHC-P, WB\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91445+Rev.+A_TDS_Purified+Anti-Human+CD45RB+(MEM-143).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry: Recommended dilution: 0.5-4 μg\/ml. The staining pattern of the MEM-143 shows prominent differences from other anti-CD45RB reagents including standard antibody MEM-55, mostly as a result of the recognition of differences in glycosylation. \u003cbr\u003eImmunohistochemistry (paraffin sections): Recommended dilution: 10 μg\/ml; positive control: human tonsil.\u003cbr\u003eWestern blotting: Recommended dilution: 1-2 μg\/ml.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eTownsend KP, et al. 2004. Neurosci Lett 362:26-30.\u003c\/li\u003e\n\u003cli\u003eLi FJ, et al. 2005. Blood 105:3295-302.\u003c\/li\u003e\n\u003cli\u003eCosenza-Nashat MA, et al. 2006. Brain Pathol 16:256-65.\u003c\/li\u003e\n\u003cli\u003eDawes R, et al. 2006. J Immunol 176:3417-25.\u003c\/li\u003e\n\u003cli\u003eBijian K, et al. 2007. Mol Immunol 44:3682-90.\u003c\/li\u003e\n\u003cli\u003eDesharnais P, et al. 2008. Apoptosis 13:197-212.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679894274084,"sku":"R7-91445","price":173.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-91445_FC_Profile.png?v=1792021230","url":"https:\/\/cytekbio.com\/products\/purified-anti-human-cd45rb-mem-143","provider":"Cytek Biosciences","version":"1.0","type":"link"}