{"product_id":"phospho-zap70-syk-tyr319-tyr352-monoclonal-antibody-n3kobu5-pe-cyanine7-ebioscience","title":"Phospho-ZAP70\/Syk (Tyr319, Tyr352) Monoclonal Antibody (n3kobu5), PE-Cyanine7, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2b, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003en3kobu5\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003ePE-Cyanine7\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu, Ms\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e5 µL (0.06 µg)\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e5 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=25-9006-42\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This n3kobu5 monoclonal antibody recognizes human and mouse zeta chain-associated protein of 70 kD (also known as ZAP-70) and spleen tyrosine kinase (also known as SYK) when phosphorylated on Y319 and Y352, respectively. ZAP-70 and SYK are members of the SYK protein tyrosine kinase (PTK) family that are phosphorylated and activated by Src family PTKs. ZAP-70\/SYK Y319\/Y352 are located in the so-called interdomain of ZAP-70\/SYK (between the N-terminal dual SH2 domains and the C-terminal kinase domain).\u003c\/p\u003e\u003cp\u003ePhosphorylation of ZAP-70 Y319 by Lck is necessary for T cell receptor (TCR)-dependent association of ZAP-70 with Lck and phospholipase C gamma and subsequent activation of calcium-dependent and Ras signaling cascades. SYK Y352 phosphorylation by Fyn\/Lyn is critical for propagation of B cell receptor (BCR) signaling and for B cell development.\u003c\/p\u003e\u003cp\u003eSpecificity of this n3kobu5 clone was determined by ELISA, flow cytometry, and western blotting.\u003c\/p\u003e\u003cp\u003eApplications Reported: This n3kobu5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This n3kobu5 antibody has been pre-diluted and tested by intracellular staining and flow cytometric analysis of sodium pervanadate-treated mouse splenocytes using the Intracellular Fixation \u0026amp; Permeabilization Buffer Set (a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/88-8824-00\"\u0026gt;Product # 88-8824-00) and protocol.  Please refer to \"Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins\" located at \u003ca href=\"https:\/\/www.thermofisher.com\/flowprotocols\"\u003ewww.thermofisher.com\/flowprotocols\u003c\/a\u003e . This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003eLight sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.\u003c\/p\u003e\u003cp\u003eFixation: Samples can be stored in IC Fixation Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-8222-49\"\u003eProduct # 00-8222-49\u003c\/a\u003e) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix\/Lyse Solution (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5333-57\"\u003eProduct # 00-5333-57\u003c\/a\u003e) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency\/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.\u003c\/p\u003e\u003cp\u003eExcitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43191007576100,"sku":"25-9006-42","price":461.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/25-9006-pzap70-sky-flow-cytometry-25apr2019lsriidrz-n3kobu5-pe-cyanine7-abmaint_20190425202428.jpg?v=1769045729","url":"https:\/\/cytekbio.com\/products\/phospho-zap70-syk-tyr319-tyr352-monoclonal-antibody-n3kobu5-pe-cyanine7-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}