{"product_id":"pacific-orange-anti-human-cd3-ucht1","title":"Pacific Orange\u003csup\u003e\u0026trade;\u003c\/sup\u003e Anti-Human CD3 (UCHT1)","description":"\u003cp\u003eThe mouse monoclonal antibody clone UCHT1 binds to human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR) and the CD3 zeta chain to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines, or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The UCHT1 binds to surface-expressed and intracellular CD3e protein, unlike an alternative human CD3 clone, HIT3a, which will stain only the extracellular (membrane-expressed) CD3e protein. A recent publication of the crystal structure of a CD3e- antibody complex provides insight as to the action of commonly used agonist antibodies, as well as specific epitope-binding data for the human CD3 antibodies UCHT1 and OKT3.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: UCHT1\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human, Chimpanzee\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Pacific Orange™\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: FC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-94909+Rev.+A_TDS_Pacific+Orange+Anti-Human+CD3+(UCHT1).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eFlow cytometry:  The suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application. The content of a vial (0.5 mL) is sufficient for 100 tests.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\u003cli\u003eFernandes, R.A. et al. 2012. J. Biol. Chem. 287: 13324-13335.\u003c\/li\u003e\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e“Pacific Orange“ is a trademark of Thermo Fisher Scientific Inc.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":67679822118948,"sku":"R7-94909","price":292.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-94909_FC_Profile.png?v=1792020406","url":"https:\/\/cytekbio.com\/products\/pacific-orange-anti-human-cd3-ucht1","provider":"Cytek Biosciences","version":"1.0","type":"link"}