{"product_id":"il-12-p35-monoclonal-antibody-4d10p35-efluor-660-ebioscience","title":"IL-12 p35 Monoclonal Antibody (4D10p35), eFluor 660, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2a, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003e4D10p35\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eeFluor 660\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eMs\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e0.125 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=50-7352-82\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This 4D10p35 monoclonal antibody reacts with mouse p35, a component of two of the four members of the interleukin (IL)-12 family of cytokines. The IL-12 family members are heterodimeric cytokines composed of an alpha-chain, such as p35, and a beta-chain, such as p40. The alpha-chain proteins are thought to be the limiting factors for secretion of IL-12 family member cytokines, as expression of the alpha-chain is more restricted than that of the beta-chain proteins, and the two subunits may need to be co-expressed in order for cytokine secretion to occur. IL-12 family members are secreted primarily by antigen-presenting cells in response to various TLR agonists and other inflammatory stimuli. The exact types of stimuli present and kinetics of stimulation determine the balance of IL-12 family member production.\u003c\/p\u003e\u003cp\u003eThe p35 protein was first identified as part of the IL-12 cytokine, which is also known as IL-12p70. Secretion is induced by IFN gamma and agonists of TLR3, 4, or 7 and is inhibited by Th2 cytokines. IL-12 is essential for the differentiation and maintenance of Th1 cells. The other subunit, p40, is expressed in much higher quantities than p35, and may be secreted independently as a monomer or homodimer. Free p40 may act as an antagonist of IL-12 function.\u003c\/p\u003e\u003cp\u003eMore recently, p35 was also found to dimerize with EBI3 (Epstein-Barr Virus-Induced Gene 3) to form IL-35. The biology of IL-35 is incompletely understood, and it is believed to be unique among the IL-12 family members in its expression and function. It is produced by regulatory T cells, and appears to have immunoregulatory functions. IL-35 has been found to suppress the proliferation and function of effector T cells, and has also been observed to induce a regulatory phenotype on naive CD4+ cells cultured in its presence.\u003c\/p\u003e\u003cp\u003eTesting of 4D10p35 in the context of IL-35 has not been evaluated. For additional information, please contact technical support.\u003c\/p\u003e\u003cp\u003eApplications Reported:  This 4D10p35 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This 4D10p35 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse dendritic cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.\u003c\/p\u003e\u003cp\u003eeFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.\u003c\/p\u003e\u003cp\u003eExcitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":43190894067748,"sku":"50-7352-82","price":452.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB_9979b573-41fc-4648-ac2c-b36fd638b9bd.jpg?v=1769042871","url":"https:\/\/cytekbio.com\/products\/il-12-p35-monoclonal-antibody-4d10p35-efluor-660-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}