{"product_id":"ikb-zeta-monoclonal-antibody-lk2nap-percp-efluor-710-ebioscience","title":"IkB zeta Monoclonal Antibody (LK2NAP), PerCP-eFluor 710, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2a, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eLK2NAP\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003ePerCP-eFluor 710\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eMs\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e0.125 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=46-6801-82\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The LK2NAP monoclonal antibody reacts with mouse IkB zeta (INAP, MAIL). IkBz is a member of the IkB family of cytoplasmic proteins and transcription factors and also shares structural homology with Bcl-3. It is induced in monocytes, macrophages and B cells in response to TLR and IL-1R signaling. In NK cells, IkBz is rapidly upregulated in response to IL-12 and IL-18 and is necessary for production of IFN gamma. In T cells, IkBz is not necessary for IFN gamma and IL-4 production by Th1 and Th2 cells, respectively. However in Th17 cells, IkBz acts in concert with RORa and RORgt to upregulate IL-17A, IL-17F, IL-21, IL-22 and IL-23R expression.\u003c\/p\u003e\u003cp\u003eApplications Reported: This LK2NAP antibody has been reported for use in intracellular staining followed by flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This LK2NAP antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated mouse splenocytes using the Foxp3\/Transcription Factor Staining Buffer Set (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5523-00\"\u003eProduct # 00-5523-00\u003c\/a\u003e) and protocol. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.\u003c\/p\u003e\u003cp\u003ePerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710\/40 band pass filter, however the 695\/40 band pass filter is an acceptable alternative. \u003c\/p\u003e\u003cp\u003eOur testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.\u003c\/p\u003e\u003cp\u003eExcitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":43190890790948,"sku":"46-6801-82","price":485.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/46-6801-82_IkB-zeta_Flow_1-20170119083539.jpg?v=1769042838","url":"https:\/\/cytekbio.com\/products\/ikb-zeta-monoclonal-antibody-lk2nap-percp-efluor-710-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}