{"product_id":"foxp3-monoclonal-antibody-nrrf-30-pe-ebioscience","title":"FOXP3 Monoclonal Antibody (NRRF-30), PE, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2a, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eNRRF-30\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003ePE\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eMs\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e0.25 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e0.2 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=12-4771-82\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The NRRF-30 antibody reacts with mouse Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead\/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+\/CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+\/CD25- cells imparts a Treg phenotype in these cells.\u003c\/p\u003e\u003cp\u003eImmunoblotting with NRRF-30 antibody has mapped the epitope to amino acids 1-75 of the mouse Foxp3 protein.\u003c\/p\u003e\u003cp\u003eIntracellular staining of mouse splenocytes with fluorochrome-conjugated NRRF-30 using the eBioscience Foxp3 Staining Buffers (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5523-00\" product\u003e) and corresponding staining protocol reveals approximately 3% of total cells in the C57Bl\/6 strain and approximately 5% in the BALB\/c mouse strain. Multicolor flow cytometric analysis demonstrates approximately 90% of the CD4+\/CD2+ cells and 4% of the CD4+\/CD25- cells staining with NRRF-30. Co-staining with FJK-16s (anti-mouse\/rat Foxp3, \u003c\/a\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/71-5775-40\"\u003eProduct # 71-5775-40\u003c\/a\u003e), which has been mapped to amino acids 71-125, and NRRF-30 shows 100% correlation, indicating that the same cells are stained with both anti-mouse Foxp3 antibodies.\u003c\/p\u003e\u003cp\u003ePlease see our FAQ regarding the usage of eBioscience Foxp3 reagents.\u003c\/p\u003e\u003cp\u003eApplications Reported: This NRRF-30 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This NRRF-30 antibody has been tested by intracellular flow cytometric analysis of mouse splenocytes using the Foxp3\/Transcription Factor Staining Buffer Set (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5523-00\"\u003eProduct # 00-5523-00\u003c\/a\u003e) and protocol. Please see \u003ca href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/references\/protocols\/cell-and-tissue-analysis\/protocols\/staining-intracellular-antigens-flow-cytometry.html?cid=bid_pca_imm_r01_co_cp1600_pgm0000_intprotocol_0wm_tfs_il_edu_an_s00_\"\u003eBestProtocols®\u003c\/a\u003e Section (Staining Intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.\u003c\/p\u003e\u003cp\u003eExcitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":43198606311460,"sku":"12-4771-82","price":528.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/12-4771-82_FOXP3_Flow_1-20170119083539.jpg?v=1769203606","url":"https:\/\/cytekbio.com\/products\/foxp3-monoclonal-antibody-nrrf-30-pe-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}