{"product_id":"cytek-universal-compbeads","title":"Cytek\u003csup\u003e®\u003c\/sup\u003e Universal CompBeads","description":"\u003cp class=\"secondary-border\" style=\"background-color: #fff; border-bottom: 2px solid #e99e2a; border-radius: 4px; box-shadow: 6px 6px 10px rgba(33, 104, 97, 0.07); padding: 16px 20px; margin: 0 0 24px; color: #e99e2a; font-size: 15px; line-height: 24px;\"\u003e\u003cstrong\u003eRecommended replacement for the discontinued product, Cytek\u003csup\u003e®\u003c\/sup\u003e FSP\u003csup\u003e®\u003c\/sup\u003e CompBeads, SKU B7-10011\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCytek\u003csup\u003e®\u003c\/sup\u003e Universal CompBeads are for use in flow cytometry. Fluorochrome-conjugated antibody binds the spherical particles for use as single-color compensation controls. They bind with mouse, rat and hamster antibodies, but also bind with rabbit and recombinant human antibodies.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003eMouse, Rat, Hamster, Human, Rabbit IgG and IgM\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eVolume Per Test:\u003c\/b\u003e\u003cspan\u003e 1 drop \/ test. Each drop contains approximately 1x10\u003csup\u003e5\u003c\/sup\u003e beads.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eApplication:\u003c\/b\u003e\u003cspan\u003e Flow cytometry\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eStorage:\u003c\/b\u003e\u003cspan\u003e 2-8°C. \u003c\/span\u003e\u003cem\u003eDo not freeze.\u003c\/em\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eData Sheets:\u003c\/b\u003e \u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/beads-buffers-solutions\/B9-50013+Rev.+A_TDS_Cytek+Universal+CompBeads+(1).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/B9-70023US+Rev.+A+Cytek%C2%AE+Universal+CompBeads%2C+100+Tests_RSD+United+States+(US)+SDS+HCS+2024+V4.15_English+(US).pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAPPLICATION\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eEach drop of beads contains two populations: a positive population that bind with any mouse, rat, hamster, rabbit or recombinant human antibody and a negative population that will not bind with antibody. When a fluorochrome-conjugated antibody is added to the beads, both positive and negative populations are observed. This bimodal distribution can be used for single-color compensation controls to accurately setup instrumentation for multicolor flow cytometry experiments.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRECOMMENDED USAGE\u003c\/strong\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cb\u003eLabel tubes:\u003c\/b\u003e Label a tube for each antibody conjugate to be used in the experiment.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eMix beads:\u003c\/b\u003e Mix the CompBeads by vigorously inverting at least 10 times or by pulse vortexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eAdd beads:\u003c\/b\u003e Add 1 drop of CompBeads to each tube (there is no need to add staining buffer).\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eAdd Antibody Conjugate:\u003c\/b\u003e Add 1 test antibody conjugate to each tube.\n\u003cp\u003e\u003cem\u003eNote:\u003c\/em\u003e A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. If high background is observed on the negative bead population, use 0.125 µg or less antibody. It is not necessary to use the antibody at its optimal concentration. For most antibodies, appropriate compensation or unmixing values result when 0.03–1.0 µg of antibody is used in the test.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003eMix the tube well by flicking or pulse-vortexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eIncubate:\u003c\/b\u003e Incubate at 2–8°C for 15–30 minutes in the dark.\u003c\/li\u003e\n\u003cli\u003e\n\u003cb\u003eWash:\u003c\/b\u003e Add 2–3 mL of Flow Cytometry Staining Buffer to each tube, then centrifuge at 400–600 × g for 3–5 minutes.\u003c\/li\u003e\n\u003cli\u003eDecant the supernatant, then add 0.2–0.4 mL of Flow Cytometry Staining Buffer to each tube.\u003c\/li\u003e\n\u003cli\u003eMix by flicking or pulse-vortexing before acquisition.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eUse appropriate personal protective equipment per the product safety data sheet when using this product.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only. Not for use in diagnostic or therapeutic procedures.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Cytek-Website","offers":[{"title":"100 tests","offer_id":51400109031460,"sku":"B7-10013","price":452.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/B7-10013_Web.png?v=1788652315","url":"https:\/\/cytekbio.com\/products\/cytek-universal-compbeads","provider":"Cytek Biosciences","version":"1.0","type":"link"}