{"product_id":"cfluor-v670-anti-human-cd86-b7-2-it2-2","title":"cFluor\u003csup\u003e\u0026reg;\u003c\/sup\u003e V670 Anti-Human CD86 (B7-2) (IT2.2)","description":"\u003cp\u003eThe mouse monoclonal antibody clone IT2.2 binds to human CD86 (B7-2), a costimulatory ligand expressed primarily on antigen-presenting cells including monocytes, dendritic cells, activated B cells, and select subsets of T cells. CD86 is a 70–80 kDa type I transmembrane glycoprotein involved in T-cell activation through engagement of CD28 and CTLA-4. Its expression is dynamically upregulated upon immune activation, making it a key marker for assessing antigen-presenting cell maturation and costimulatory capacity. Clone IT2.2 recognizes an extracellular epitope of CD86 and is validated for flow cytometry to assess activation status in immune monitoring, immuno-oncology, and dendritic-cell assays.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: IT2.2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD86 (B7-2)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: cFluor\u003csup\u003e®\u003c\/sup\u003e V670\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2b, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Flow Cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light. \u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/R8-51339+Rev.+A_TDS_cFluor+V670+Anti-Human+CD86+(B7-2)+(IT2.2).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.\u003cbr\u003e\u003cbr\u003ePlease briefly centrifuge the reagent vial before use.\u003cbr\u003e\u003cbr\u003eUse appropriate personal protective equipment per the product safety data sheet when using this product.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eFreeman GJ, Gray GS, Gimmi CD, et al. 1993. Science. 262:909–911.\u003c\/li\u003e\n\u003cli\u003eHathaway WE, Bondurant M, Foy TM, et al. 1994. J Immunol Methods. 170:145–155.\u003c\/li\u003e\n\u003cli\u003eGuilliams M, Bruhns P, Saeys Y, et al. 2014. Nat Rev Immunol. 14:571–587.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003ecFluor\u003csup\u003e®\u003c\/sup\u003e V670 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.\u003c\/p\u003e\u003cp\u003eCytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e V670 is StarBright\u003csup\u003e™\u003c\/sup\u003e Violet 670 Dye, manufactured and provided by Bio-Rad, Inc.\u003c\/p\u003eStarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43582719033380,"sku":"R7-21339","price":535.0,"currency_code":"USD","in_stock":true},{"title":"25 Tests","offer_id":43582719066148,"sku":"R7-21340","price":214.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/CD86_IT2.2_V670_c1aee87b-a339-4ec8-aa24-c11ce310dace.png?v=1771873342","url":"https:\/\/cytekbio.com\/products\/cfluor-v670-anti-human-cd86-b7-2-it2-2","provider":"Cytek Biosciences","version":"1.0","type":"link"}