The HI30 monoclonal antibody binds to isoforms of human CD45, a 180-240-kDa type I membrane glycoprotein, also known as LCA (leukocyte common antigen). CD45 is the first and prototypic receptor-like protein tyrosine phosphatase that expresses on all human leukocytes^1,2. It is absent on mature erythrocytes, platelets and non-hematopoietic cells. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  HI30

Reactivity:          Human, Chimpanzee

Format:                cFluor® V547¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

REFERENCES

1. Terry LA, et al. 1990. Immunology. 64:331
2. Hermiston ML, et al. 2003. Annu Rev Immunol. 21:107

This product is intended for in vitro diagnostic use to identify human cells expressing CD45 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

¹cFluor® V547 is equivalent to CF®405L manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.