{"product_id":"cfluor-v515-anti-human-cd195-ccr5-hek-1-85a","title":"cFluor\u003csup\u003e\u0026reg;\u003c\/sup\u003e V515 Anti-Human CD195 (CCR5) (HEK\/1\/85a)","description":"\u003cp\u003eThe rat monoclonal antibody clone HEK\/1\/85a binds to human CD195, also known as CCR5. CCR5 is a 45 kDa G protein-coupled receptor (GPCR) featuring seven transmembrane domains. It belongs to the beta chemokine receptor family and is primarily expressed on subsets of T lymphocytes (particularly CD3+CD45RO+CD95+ cells) and monocytes. CCR5 binds several chemokines, notably MIP-1α, MIP-1β, RANTES, and MCP-2, triggering intracellular signaling pathways essential for cell differentiation, proliferation, chemotaxis, lymphocyte activation, and transendothelial migration during inflammatory responses. CCR5 is also recognized as a co-receptor necessary for the entry of macrophage-tropic HIV-1 (R5 HIV-1) into CD4+ immune cells. Post-translational modifications, such as sulfation, significantly influence HIV entry efficiency. Genetic studies highlight that individuals with partial or complete CCR5 gene deletions exhibit marked resistance to HIV infection. Additionally, CCR5 is implicated in various human diseases, including infections, inflammation, and cancers.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: HEK\/1\/85a\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD195 (CCR5)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: cFluor\u003csup\u003e®\u003c\/sup\u003e V515\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rat IgG2a, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µL\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Flow Cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light. \u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/R8-90109+Rev.+A_TDS_cFluor+V515+Anti-Human+CD195+(CCR5)+(HEK185a).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.\u003cbr\u003e\u003cbr\u003ePlease briefly centrifuge the reagent vial before use.\u003cbr\u003e\u003cbr\u003eUse appropriate personal protective equipment per the product safety data sheet when using this product.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003ecFluor\u003csup\u003e®\u003c\/sup\u003e V515 is a tandem dye. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.\u003c\/p\u003e\u003cp\u003eCytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e V515 is StarBright\u003csup\u003e™\u003c\/sup\u003e Violet 515 Dye, manufactured and provided by Bio-Rad, Inc.\u003c\/p\u003eStarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":44228277338148,"sku":"R7-90109","price":492.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-90109.png?v=1780510553","url":"https:\/\/cytekbio.com\/products\/cfluor-v515-anti-human-cd195-ccr5-hek-1-85a","provider":"Cytek Biosciences","version":"1.0","type":"link"}