cFluor® V450 Anti-Human CD14 (M5E2)
The M5E2 monoclonal antibody binds to human CD14, a 53-55-kDa glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that works as a receptor on myeloid cells for ligands such as lipopolysaccharide (LPS)1. It is found that CD14 is a receptor for and binds to complexes of LPS and LBP with high affinity2. It expresses on monocytes and macrophages at high levels. It is also present in some interfollicular dendritic cells and macrophages, reticular dendritic cells and Langerhans cells. The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Reactivity: Human, Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cow, Cynomolgus, Dog, Pigtailed Macaque, Rhesus, Squirrel Monkey
Format: cFluor® V450
Isotype: Mouse IgG2a, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).
- Pugin J, et al. 1998. Infect Immun. 66:1174
- Wright SD, et al. 1990. Science. 249:1431
This product is intended for in vitro diagnostic use to identify human cells expressing CD14 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.