{"product_id":"cfluor-uv395-anti-mouse-cd4-rm4-5","title":"cFluor\u003csup\u003e\u0026reg;\u003c\/sup\u003e UV395 Anti-Mouse CD4 (RM4-5)","description":"\u003cp\u003eThe rat monoclonal antibody clone RM4 binds to mouse CD4, a 55 kDa protein that acts as a co-receptor for the T cell receptor (TCR) in its interaction with major histocompatibility complex (MHC) class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta2-domain of MHC class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase Lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, and on dendritic cells. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will \"compete\" for binding, i.e., the RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, the RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: RM4-5\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD4\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: cFluor\u003csup\u003e®\u003c\/sup\u003e UV395\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rat IgG2a, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µl\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Flow Cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light. \u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/R8-50811+Rev.+C_TDS_cFluor+UV395+Anti-Mouse+CD4+(RM4-5).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.\u003cbr\u003e\u003cbr\u003ePlease briefly centrifuge the reagent vial before use.\u003cbr\u003e\u003cbr\u003eUse appropriate personal protective equipment per the product safety data sheet when using this product.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003eCytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e UV395 is StarBright\u003csup\u003e™\u003c\/sup\u003e UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.\u003c\/p\u003eStarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43561797615652,"sku":"R7-20811","price":502.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-20811.png?v=1771017580","url":"https:\/\/cytekbio.com\/products\/cfluor-uv395-anti-mouse-cd4-rm4-5","provider":"Cytek Biosciences","version":"1.0","type":"link"}