{"product_id":"cfluor-uv395-anti-human-mouse-cd45r-b220-ra3-6b2","title":"cFluor\u003csup\u003e\u0026reg;\u003c\/sup\u003e UV395 Anti-Human\/Mouse CD45R (B220) (RA3-6B2)","description":"\u003cp\u003eThe rat monoclonal antibody clone RA3-6B2 binds to the human and mouse CD45 isoform known as CD45R, or B220, a 220 kDa protein tyrosine phosphatase. CD45 is one of the most abundant hematopoietic markers expressed on all leukocytes (leukocyte common antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mice, the CD45R\/B220 isoform is predominantly found on B cells at varying levels in all stages, from pro-B cells to activated B cells, and may also be detected on specific T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO, and several others. The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets, and NK cell subsets in human and mouse.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: RA3-6B2\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: CD45R (B220)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human, Mouse, Chimpanzee\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: cFluor\u003csup\u003e®\u003c\/sup\u003e UV395\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Rat IgG2a, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution\u003c\/b\u003e: 5 µl\/Test\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: Flow Cytometry\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light. \u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate-buffered saline, pH 7.2, containing 0.1% PEG 3350, 0.1% Pluronic F-68, 0.05% Tween-20, 0.09% sodium azide and 1% BSA (BSA Country of Origin USA)\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/R8-50813+Rev.+C_TDS_cFluor+UV395+Anti-Human+Mouse+CD45R+(B220)+(RA3-6B2).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eEach lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 μL per 1 million cells in a staining volume of 100 μL. If whole blood is analyzed, then use 5 μL per 100 μL. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.\u003cbr\u003e\u003cbr\u003ePlease briefly centrifuge the reagent vial before use.\u003cbr\u003e\u003cbr\u003eUse appropriate personal protective equipment per the product safety data sheet when using this product.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003eCytek\u003csup\u003e®\u003c\/sup\u003e cFluor\u003csup\u003e®\u003c\/sup\u003e UV395 is StarBright\u003csup\u003e™\u003c\/sup\u003e UltraViolet 400 Dye, manufactured and provided by Bio-Rad, Inc.\u003c\/p\u003eStarBright is a trademark of Bio-Rad Laboratories, Inc. in certain jurisdictions. StarBright Dyes are manufactured and provided by Bio-Rad under an Agreement between Bio-Rad supplied to Cytek, being subject to certain conditions.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43561797779492,"sku":"R7-20813","price":502.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/R7-20813.png?v=1771017586","url":"https:\/\/cytekbio.com\/products\/cfluor-uv395-anti-human-mouse-cd45r-b220-ra3-6b2","provider":"Cytek Biosciences","version":"1.0","type":"link"}