The L17F12 monoclonal antibody binds to human CD5, a 67-kDa single chain type I glycoprotein in the scavenger receptor family ⁽¹⁾⁽²⁾.  CD5, also known as Leu-1, Ly-1 or T1, is expressed on thymocytes, T cells and subsets of B cells. CD5 binds to its ligand on B cells, CD72 (LYB-2), to play a role in T-B cell interaction and to modulate the intensity of antigen receptor signal transduction ⁽³⁾. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  L17F12

Reactivity:          Human

Format:              cFluor® R720¹

Isotype:               Mouse IgG2a, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze.

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

REFERENCES

1. Engleman EG, et al. 1981. Proc Natl Acad Sci USA. 78(3):1791-5
2. Velasco-de Andrés, María et al. 2020. Cells vol. 9,12 2589
3. Tabbekh, Mouna et al. 2013. Oncoimmunology 2,1

This product is intended for in vitro diagnostic use to identify human cells expressing CD5 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

¹cFluor® R720 is equivalent to CF®700 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel.