The 3G8 monoclonal antibody binds to IgG receptor III (FcγRIII) that are in two forms: CD16a ((FcγRIIIA) and CD16b ((FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kD polypeptide-anchored transmembrane protein and a 48 kD GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages while CD16b is expressed on neutrophils^1,2. CD16a also plays a crucial role for antibody-dependent cellular cytotoxicity (ADCC) by NK cells³. The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  3G8

Reactivity:          Human, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey

Format:               cFluor® R668¹

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:      Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (BSA Country of Origin USA).

Data Sheets:       IFU SDS

REFERENCES

1. Wirthmueller U, et al. 1992. J Exp Med. 175:1381
2. Smed-Sörensen A, et al. 2008. Blood. 111:5037
3. Wei H Y, et al, 2016. Sci Rep. 6:34310

This product is intended for in vitro diagnostic use to identify human cells expressing CD16 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.

 ¹cFluor® R668 is equivalent to CF®647 manufactured and provided by Biotium, Inc. under an Agreement between Biotium and Cytek (LICENSEE). The manufacture, use, sale, offer for sale, or import of the product is covered by one or more of the patents or pending applications owned or licensed by Biotium. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel