cFluor® BYG575 Anti-Human CD16 (3G8)
The 3G8 monoclonal antibody binds to IgG receptor III (FcγRIII) that are in two forms: CD16a ((FcγRIIIA) and CD16b ((FcγRIIIB). With 95% sequence similarity, they are a conventional 50-65 kD polypeptide-anchored transmembrane protein and a 48 kD GPI-anchored protein, respectively. CD16a is expressed on NK cells and macrophages while CD16b is expressed on neutrophils1,2. CD16a also plays a crucial role for antibody-dependent cellular cytotoxicity (ADCC) by NK cells3. The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Reactivity: Human, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel
Format: cFluor® BYG575
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
- Wirthmueller U, et al. 1992. J Exp Med. 175:1381
- Smed-Sörensen A, et al. 2008. Blood. 111:5037
- Wei H Y, et al, 2016. Sci Rep. 6:34310
This product is intended for in vitro diagnostic use to identify human cells expressing CD16 antigen molecules in countries where the regulatory approval has been obtained from the local regulatory authorities.