Shopping Cart
cFluor® B690 anti-Human Lambda Light Chain (1-155-2)

cFluor® B690 anti-Human Lambda Light Chain (1-155-2)

SKU R7-20260
Regular price

$110.00

task_alt Added to cart.
View Cart or Continue shopping.
Description

The 1-155-2 monoclonal antibody binds to human immunoglobulin light chain, lambda (λ), a 22.5-kDa polypeptide subunit of an antibody. There are two types of immunoglobulin light chains in human: kappa (κ) and lambda (λ). Under normal condition, antibodies produced by B cells only express one type of light chain and ratio of kappa to lambda is approximately 2:1(3)(4). The antibody was conjugated to a fluorophore and purified by affinity chromatography.

PRODUCT DETAILS

Clone:                  1-155-2

Reactivity:         Human

Format:              cFluor® B6901

Isotype:               Mouse IgG1, κ

Tested Dilution:  5 μL / test

Application:        Flow Cytometry

Storage:               2-8°C and protected from Light. Do not freeze

Formulation:       Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)

Data Sheets:       TDS SDS

 

RECOMMENDED USAGE

Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL of washed blood. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.

 Please briefly centrifuge the reagent vial before use.

Use appropriate personal protective equipment per the product safety data sheet when using this product.

 

Spectral signature of cFluor® B690 from a Cytek® Aurora 5 laser system equipped with 355 nm, 405 nm, 488 nm, 561 nm and 640 nm lasers using CytekAssaySettings..

REFERENCES

  1. Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
  2. Kern Petra S. et al. 1998. Immunity. 519-530
  3. Veillette A. et al. 1988. Cell. 55:301-308

 

These products are for research use only. Not intended for use in diagnostic procedures.

 

 

1cFluor® B690 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.

Shipping
Price calculated at checkout.