cFluor® B690 anti-Human Lambda Light Chain (1-155-2)
The 1-155-2 monoclonal antibody binds to human immunoglobulin light chain, lambda (λ), a 22.5-kDa polypeptide subunit of an antibody. There are two types of immunoglobulin light chains in human: kappa (κ) and lambda (λ). Under normal condition, antibodies produced by B cells only express one type of light chain and ratio of kappa to lambda is approximately 2:1(3)(4). The antibody was conjugated to a fluorophore and puriﬁed by afﬁnity chromatography.
Format: cFluor® B6901
Isotype: Mouse IgG1, κ
Tested Dilution: 5 μL / test
Application: Flow Cytometry
Storage: 2-8°C and protected from Light. Do not freeze
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% BSA (Origin USA)
Each lot of this antibody is quality control tested using flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per 1 million cells in a staining volume of 100 µL. If whole blood is analyzed, then use 5 µL per 100 µL of washed blood. It is recommended that users titrate the antibody to obtain the optimal result for their specific application.
Please briefly centrifuge the reagent vial before use.
Use appropriate personal protective equipment per the product safety data sheet when using this product.
Spectral signature of cFluor® B690 from a Cytek® Aurora 5 laser system equipped with 355 nm, 405 nm, 488 nm, 561 nm and 640 nm lasers using CytekAssaySettings..
- Cole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8
- Kern Petra S. et al. 1998. Immunity. 519-530
- Veillette A. et al. 1988. Cell. 55:301-308
These products are for research use only. Not intended for use in diagnostic procedures.
1cFluor® B690 is a tandem dye made with PerCP. Caution – Tandem dyes may show changes in their emission spectra with prolonged exposure to light or fixatives.