{"product_id":"cd3-cd4-cd8a-antibody-cocktail-apc-fitc-percp-efluor-710-ebioscience","title":"CD3\/CD4\/CD8a Antibody Cocktail, APC, FITC, PerCP-eFluor 710, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG1, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eCocktail\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eSK3, SK7, SK1\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eAPC\/FITC\/PerCP-eFluor 710\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e20 µL\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e20 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=22-0306-71\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: This three-color cocktail is designed to identify T cells from peripheral blood samples. It can be used alone, or in combination with other antibodies to further characterize T cell populations. The SK3 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The SK7 monoclonal antibody reacts with human CD3 eplison, a 20 kDa subunit of the CD3\/TCR complex. The CD3 complex, including the epsilon, gamma and delta subunits, is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8beta chain (CD8alpha beta) or as a homodimer (CD8alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. \u003c\/p\u003e\u003cp\u003eThis cocktail contains the following antibodies:\u003c\/p\u003e\u003cp\u003eAnti-Human CD4 (SK3) FITC\u003c\/p\u003e\u003cp\u003eAnti-Human CD3 (SK7) PerCP-eFluor™ 710\u003c\/p\u003e\u003cp\u003eAnti-Human CD8a (SK1) APC\u003c\/p\u003e\u003cp\u003eApplications Reported:  eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been reported for use in flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: eZfluor Anti-Human CD4 FITC, CD3 PerCP-eFluor™ 710, CD8a APC has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 µL per test, which contains 0.06 µg of Anti-Human CD4 FITC, 0.06 µg of Anti-Human CD3 PerCP-eFluor™ 710, and 0.25 µg of Anti-Human CD8a APC. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"25 Tests","offer_id":43188413595684,"sku":"22-0306-71","price":394.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/22-0306-71-CD3-CD4-CD8a-Flow-20170216130641.jpg?v=1769023482","url":"https:\/\/cytekbio.com\/products\/cd3-cd4-cd8a-antibody-cocktail-apc-fitc-percp-efluor-710-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}