{"product_id":"cd223-lag-3-monoclonal-antibody-3ds223h-brilliant-ultra-violet-805-ebioscience","title":"CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Ultra Violet 805, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG1, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003e3DS223H\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eBrilliant Ultra Violet 805\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e5 µL (0.5 µg)\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e5 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=368-2239-41\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).\u003c\/p\u003e\u003cp\u003eThis 3DS223H antibody will recognize a formaldehyde-fixed epitope.\u003c\/p\u003e\u003cp\u003eApplications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.\u003c\/p\u003e\u003cp\u003eApplications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003eBrilliant Ultra Violet™ 805 (BUV805) is a tandem dye that emits at 797 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.\u003c\/p\u003e\u003cp\u003eWhen using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/SB-4401-42\"\u003eProduct # SB-4401\u003c\/a\u003e) or Brilliant Stain Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-4409-75\"\u003eProduct # 00-4409-75\u003c\/a\u003e) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.\u003c\/p\u003e\u003cp\u003eLight sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.\u003c\/p\u003e\u003cp\u003eFixation: Samples can be stored in IC Fixation Buffer (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-8222-49\"\u003eProduct # 00-8222\u003c\/a\u003e) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix\/Lyse Solution (\u003ca href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/00-5333-54\"\u003eProduct # 00-5333\u003c\/a\u003e) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency\/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.\u003c\/p\u003e\u003cp\u003eOur internal testing suggests that Brilliant Ultra Violet™ 805 (BUV805) is compatible with short-term methanol-based fixation, but should not be stored in buffers containing methanol for longer than one hour.\u003c\/p\u003e\u003cp\u003eExcitation: 355 nm; Emission: 797 nm; Laser: Ultraviolet Laser.\u003c\/p\u003e\u003cp\u003eBRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"25 Tests","offer_id":43182148943908,"sku":"368-2239-41","price":224.0,"currency_code":"USD","in_stock":true},{"title":"100 Tests","offer_id":43182148976676,"sku":"368-2239-42","price":410.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/368-2239-cd223lag-3-flow-cytometry-19apr2021aurje-3ds223h-buv805-abmaint_20210503194253.jpg?v=1768960349","url":"https:\/\/cytekbio.com\/products\/cd223-lag-3-monoclonal-antibody-3ds223h-brilliant-ultra-violet-805-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}