{"product_id":"cd133-prominin-1-monoclonal-antibody-emk08-fitc-ebioscience","title":"CD133 (Prominin-1) Monoclonal Antibody (EMK08), FITC, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eMouse\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2b, kappa\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eEMK08\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eFITC\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eHu\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e5 µL (0.06 µg)\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e5 μL\/Test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C, store in dark, DO NOT FREEZE!\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with BSA and 0.09% sodium azide\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=11-1339-42\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eThe EMK08 monoclonal antibody reacts with human CD133 (Prominin-1), a 120 kDa member of the pentaspan family of proteins, which also includes Prominin-2. Their expression is found within plasma membrane protrusions such as epithelial microvilli. CD133 can exist in a number of alternatively spliced isoforms, and the protein has several N-linked glycosylation sites; the occurrence of both may be tissue-dependent. Human CD133 was first identified as an epitope expressed on CD34+ hematopoietic progenitors. Although the ligand and function of CD133 remain unknown, it has since proven to be very useful as a marker for both stem cells and cancer stem cells. In addition to its expression on hematopoietic precursors, CD133 has been used to identify tumorigenic colon cancer stem cells, brain cancer stem cells, prostate cancer stem cells, in addition to others. \u003c\/p\u003e\u003cp\u003eThe binding of the EMK08 antibody does not block the binding of another anti-human CD133 antibody, TMP4 (\u003ca href=\"https:\/\/www.thermofisher.com\/antibody\/product\/CD133-Prominin-1-Antibody-clone-TMP4-Monoclonal\/12-1338-42\"\u003eProduct # 12-1338-42\u003c\/a\u003e) indicating that they recognize distinct epitopes.\u003c\/p\u003e\u003cp\u003eThis EMK08 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"100 Tests","offer_id":43181905444900,"sku":"11-1339-42","price":432.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/11-1339-42_CD133_Flow_1_20170217141030.jpg?v=1768945353","url":"https:\/\/cytekbio.com\/products\/cd133-prominin-1-monoclonal-antibody-emk08-fitc-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}