{"product_id":"cd126-monoclonal-antibody-d7715a7-functional-grade-ebioscience","title":"CD126 Monoclonal Antibody (D7715A7), Functional Grade, eBioscience","description":"\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost: \u003c\/b\u003e\u003cspan\u003eRat\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype: \u003c\/b\u003e\u003cspan\u003eIgG2b\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClonality: \u003c\/b\u003e\u003cspan\u003eMonoclonal\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone: \u003c\/b\u003e\u003cspan\u003eD7715A7\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat: \u003c\/b\u003e\u003cspan\u003eFunctional Grade\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity: \u003c\/b\u003e\u003cspan\u003eMs\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication: \u003c\/b\u003e\u003cspan\u003eFlow Cytometry\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eTested Dilution: \u003c\/b\u003e\u003cspan\u003e1 µg\/test\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eConcentration: \u003c\/b\u003e\u003cspan\u003e1 mg\/mL\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage: \u003c\/b\u003e\u003cspan\u003e4°C\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation: \u003c\/b\u003e\u003cspan\u003ePBS with no preservative; pH 7.2\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003ePurification: \u003c\/b\u003e\u003cspan\u003eAffinity chromatography\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheet: \u003c\/b\u003e\u003ca href=\"https:\/\/www.thermofisher.com\/order\/genome-database\/dataSheetPdf?producttype=antibody\u0026amp;productsubtype=antibody_primary\u0026amp;productId=16-1261-85\" target=\"_blank\"\u003eTDS\u003c\/a\u003e\u003c\/p\u003e \u003cbr\u003e\u003cp\u003e\u003cspan\u003e\u003cb\u003eSpecific Information\u003c\/b\u003e\u003c\/span\u003e\u003c\/p\u003e\u003cp\u003eDescription: The monoclonal antibody D7715A7 recognizes mouse CD126, the IL-6 receptor alpha chain, a member of the Ig superfamily. CD126 can heterodimerize with CD130 (gp130) to form the high affinity IL-6 receptor. Once IL-6 is bound, signaling from the CD130 subunit is initiated and the receptor complex is internalized. The IL-6 receptor is also reported to exist as a soluble version, which bound to IL-6 acts in trans to signal. Expression is found on T lymphocytes in the periphery and during development in the thymus is restricted to SP populations, unlike gp130 which is expressed in all thymocytes. Additional expression has been shown on activated B lymphocytes, macrophages, granulocytes and CD34+ hematopoietic progenitor cells. The monoclonal antibody D7715A7 binding can be blocked addition of ligand (IL-6).\u003c\/p\u003e\u003cp\u003eApplications Reported:  This D7715A7 antibody has been reported for use in flow cytometric analysis and has also been reported to block cytokine binding and inhibit in vitro and in vivo growth of cells dependent on IL-6.\u003c\/p\u003e\u003cp\u003eApplications Tested: This D7715A7 antibody has been tested by blocking of direct conjugate when staining mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells\/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.\u003c\/p\u003e\u003cp\u003eStorage and handling: Use in a sterile environment.\u003c\/p\u003e\u003cp\u003eFiltration: 0.2 µm post-manufacturing filtered.\u003c\/p\u003e\u003cp\u003ePurity: Greater than 90%, as determined by SDS-PAGE.\u003c\/p\u003e\u003cp\u003eEndotoxin Level:  Less than 0.001 ng\/µg antibody, as determined by LAL assay.\u003c\/p\u003e\u003cp\u003eAggregation:  Less than 10%, as determined by HPLC.\u003c\/p\u003e\u003cp\u003e \u003c\/p\u003eFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.","brand":"Cytek Biosciences","offers":[{"title":"500 µg","offer_id":43181900300324,"sku":"16-1261-85","price":438.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/16-1261-85_CD126_Flow_1-20170119083539.jpg?v=1768945248","url":"https:\/\/cytekbio.com\/products\/cd126-monoclonal-antibody-d7715a7-functional-grade-ebioscience","provider":"Cytek Biosciences","version":"1.0","type":"link"}