{"product_id":"alexa-fluor-488-anti-lck-phospho-tyr394-a18002d","title":"Alexa Fluor® 488 anti-Lck Phospho (Tyr394)","description":"\u003cp\u003eThe Src family tyrosine kinase p56Lck(Lck) is a non-receptor tyrosine kinase that plays a critical role in T cell selection and maturation within the thymus, and also in the function of mature T cells. Lck, which is constitutively bound to cytosolic domains of CD4 and CD8 surface receptors, plays an essential role in T cell receptor (TCR) signaling. Engagement of the TCR with peptide antigen-loaded MHC complexes results in the recruitment of CD4- and CD8-bound Lck to the TCR\/CD3 signaling complex. Lck then transphosphorylates TCR-gamma chains and CD3 subunits, thereby activating the TCR\/CD3 signaling pathway and leading to the recruitment and subsequent phosphorylation of Zap70 by Lck. Lck also plays an important role in interleukin-2 signaling that regulates the T cell proliferative response. Phosphorylation of Lck by CSK at tyrosine 505 negatively regulates the kinase, and is proposed to generate a closed, inactive conformation of the protein. Conversely, phosphorylation at tyrosine 394 in the activation loop of Lck greatly stimulates enzymatic activity. In many types of cancer, Lck is a proliferative and anti-apoptotic driver, and has been proposed as a target for therapeutic intervention.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone:\u003c\/b\u003e A18002D\u003c\/p\u003e\u003cp\u003e\u003cb\u003eAntibody Type:\u003c\/b\u003e Monoclonal\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity:\u003c\/b\u003e Human, Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat:\u003c\/b\u003e Lck Phospho Tyr394\u003c\/p\u003e\u003cp\u003e\u003cb\u003eHost:\u003c\/b\u003e Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype:\u003c\/b\u003e IgG1, κ\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplications:\u003c\/b\u003e ICFC - Quality tested ICC - Verified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eRegulatory Status:\u003c\/b\u003e RUO\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eData Sheets:\u003c\/strong\u003e \u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/biolegend\/alexa-fluor-488-anti-lck-phospho-tyr394-a18002d-27235+(TDS).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/biolegend\/Pure_Conjugated_Antibodies_SDS_United_States_English_V2.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eNote to Purchaser: \u003c\/strong\u003eThis product is manufactured and supplied by BioLegend, Inc. under an agreement between BioLegend, Inc. and Cytek Biosciences, Inc. Information related to this product is provided by BioLegend, Inc.\u003c\/p\u003e","brand":"BioLegend","offers":[{"title":"100 μg","offer_id":51193997951012,"sku":"933108","price":360.0,"currency_code":"USD","in_stock":true},{"title":"25 μg","offer_id":51193997983780,"sku":"933107","price":160.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/cytekbio.com\/products\/alexa-fluor-488-anti-lck-phospho-tyr394-a18002d","provider":"Cytek Biosciences","version":"1.0","type":"link"}