{"product_id":"purified-anti-mouse-foxp3-3g3","title":"Purified Anti-Mouse Foxp3 (3G3)","description":"\u003cp\u003eThe mouse monoclonal antibody clone 3G3 binds to mouse Foxp3, a 50-55 kDa transcription factor, which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted and, upon TCR activation, it is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce the expression of Foxp3 in naive T cells, driving their development into Foxp3+ Tregs, which are called induced or adaptive Tregs. These cells are phenotypically similar to so-called natural Tregs (CD4+ CD25high Foxp3+), which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity. Forced expression of Foxp3 in conventional T cells results in the upregulation of Treg-associated molecules such as CD25, CTLA-4, and GITR, and is sufficient to impart suppressive functional activity to these cells. It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed.\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: 3G3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: Foxp3\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG1, k\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: WB, FC-IC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91695+Rev.+A_TDS_Purified+Anti-Mouse+Foxp3+(3G3).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eWestern blotting: Recommended dilution: 2 μg\/ml. \u003cbr\u003eFlow cytometry: Recommended dilution: 1-4 µg\/ml. Intracellular staining.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eCole DK, et al. 2004. Cell Mol Immunol. 1(2):81-8.\u003c\/li\u003e\n\u003cli\u003eKern Petra S, et al. 1998. Immunity. 9: 519-530.\u003c\/li\u003e\n\u003cli\u003eVeillette A. et al. 1988. Cell. 55:301-308.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679927926820,"sku":"R7-91695","price":279.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB.jpg?v=1613537357","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-mouse-foxp3-3g3","provider":"Cytek Biosciences","version":"1.0","type":"link"}