{"product_id":"purified-anti-human-mouse-fyn-fyn-01","title":"Purified Anti-Human\/Mouse FYN (FYN-01)","description":"\u003cp\u003eThe mouse monoclonal antibody FYN-01 recognizes Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase (intracellular antigen).\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003ePRODUCT DETAILS\u003c\/strong\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eClone\u003c\/b\u003e: FYN-01\u003c\/p\u003e\u003cp\u003e\u003cb\u003eSpecificity\u003c\/b\u003e: FYN\u003c\/p\u003e\u003cp\u003e\u003cb\u003eReactivity\u003c\/b\u003e: Human|Mouse\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormat\u003c\/b\u003e: Purified\u003c\/p\u003e\u003cp\u003e\u003cb\u003eIsotype\u003c\/b\u003e: Mouse IgG2b\u003c\/p\u003e\u003cp\u003e\u003cb\u003eApplication\u003c\/b\u003e: IP, WB, IHC-P, ICC\u003c\/p\u003e\u003cp\u003e\u003cb\u003eStorage\u003c\/b\u003e: 2-8°C protected from light.\u003cb\u003eDo not freeze\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003e\u003cb\u003eFormulation\u003c\/b\u003e: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide\u003c\/p\u003e\u003cp\u003e\u003cb\u003eData Sheets: \u003c\/b\u003e\u003ca href=\"https:\/\/cytek-web.s3.amazonaws.com\/cytekbio.com\/documentation-center\/TDS\/cfluor\/project_elva_2k\/R8-91425+Rev.+A_TDS_Purified+Anti-HumanMouse+FYN+(FYN-01).pdf\" target=\"_blank\"\u003eTDS\u003c\/a\u003e | \u003ca href=\"https:\/\/cytek-web.s3.us-east-1.amazonaws.com\/cytekbio.com\/documentation-center\/SDS\/R9-70004+Rev.+E_SDS+fluorescent+conjugated+Abs.pdf\" target=\"_blank\"\u003eSDS\u003c\/a\u003e\u003c\/p\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eRECOMMENDED USAGE\u003c\/b\u003e\u003c\/p\u003e\u003cp\u003eImmunohistochemistry (paraffin sections): Recommended dilution: 5 μg\/ml; positive tissue: brain. \u003cbr\u003eWestern blotting: Recommended dilution: 2 μg\/ml; positive control: JURKAT human T cell leukemia cell line. \u003cbr\u003eImmunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris\/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w\/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.\u003c\/p\u003e\u003cbr\u003e\u003cbr\u003e\u003cp\u003e\u003cb\u003eREFERENCES\u003c\/b\u003e\u003c\/p\u003e\u003col\u003e\n\u003cli\u003eRivera J, et al. 2008. Curr Allergy Asthma Rep 8:14-20.\u003c\/li\u003e\n\u003cli\u003eMakinodan E, et al. 2007. Neuroscience 147:1-4.\u003c\/li\u003e\n\u003cli\u003eSamayawardhena LA, et al. 2007. Blood 109:3679-86.\u003c\/li\u003e\n\u003cli\u003eFilby A, et al. 2007. J Immunol 179:4635-44.\u003c\/li\u003e\n\u003cli\u003eWu HY, et al. 2007. J Biol Chem 282:20075-87.\u003c\/li\u003e\n\u003cli\u003eHattori K, et al. 2006. J Biol Chem 281:7129-35.\u003c\/li\u003e\n\u003cli\u003eTalmor-Cohen A, et al. 2004. Reproduction 128:387-93.\u003c\/li\u003e\n\u003cli\u003eUtting O, et al. 2001. J Immunol 166:1540-6.\u003c\/li\u003e\n\u003cli\u003eMeraner P, et al. 2007. J Immunol 178:2262-71.\u003c\/li\u003e\n\u003c\/ol\u003e\u003cbr\u003e\u003cp\u003e\u003cstrong\u003eFor research use only. Not intended for use in diagnostic procedures.\u003c\/strong\u003e\u003c\/p\u003e\u003cbr\u003e","brand":"Cytek Biosciences","offers":[{"title":"100 µg","offer_id":67679920554020,"sku":"R7-91425","price":239.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1654\/6801\/files\/Symbol_Full_Color_-_JPG_RGB.jpg?v=1613537357","url":"https:\/\/cytekbio.com\/ja\/products\/purified-anti-human-mouse-fyn-fyn-01","provider":"Cytek Biosciences","version":"1.0","type":"link"}